DNA fingerprinting (VNTR)डीएनए अंगुलिछापी (VNTR)

NCERT Fig 5.16 · Class 12 Biology · Chapter 5 · interactive diagram

Parts and their functions

• Chromosomes carrying VNTRs (VNTR वाले गुणसूत्र) — DNA is isolated from any tissue — blood, hair follicle, skin, bone, saliva, sperm.
• VNTR (mini-satellite) (VNTR (मिनी-सैटेलाइट)) — Short sequence repeated in tandem; copy number varies, size 0.1–20 kb.
• Autoradiogram (ऑटोरेडियोग्राम) — After restriction digestion, electrophoresis, blotting and hybridisation with a labelled VNTR probe.
• Band pattern (पट्टी पैटर्न) — Bands of different sizes — a pattern unique to each person.
• Monozygotic twins (एकयुग्मजी जुड़वाँ) — The only people with the same pattern.

Steps

1. Different people carry different numbers of VNTR repeats.
2. Cut, separate, blot, probe → band pattern on the autoradiogram.
3. Every person differs except identical twins.

Original diagram drawn by Simple Learning Hub, based on NCERT Fig 5.16. Report a mistake